R784W (LQT-2)

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Voltage dependence of tail current Current density of tail current @-40mv Expression System Other Reference
WT
1.5±0.8 mV
W.T.
37.6±6.1 pA/pF 
CHO dominant negative effect not tested Yang et al 2002
RW
14.8 ±0.4 mV
RW
9.5 ±1.1pA/pF
co-exp. with KCNE1    
Positive 75% reduction       

COMMENTS ON IN VITRO DATA
R784W mutation has found in a patient with amiodarone-induced QT interval prolongation. R784W has been identified in 1/92 patients with drug-induced Torsades de Pointes by Yang et al 2002, in a population-based study aimed quantifying the prevalence of LQTS-related gene mutations in such group of patients. In vitro expression showed that this mutation reduces IKr currents but do not abolish them, thus suggesting a possible direct correlation with the mild clinical phenotype similarly to KvR583C mutation. The presence of a dominant negative effect was not tested. .

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